Slu-PP-332 10mg

SLU-PP-332 is a synthetic small molecule (non-peptide) of the acylhydrazone class, chemically a 4-hydroxybenzohydrazide derivative with a naphthalene group. In scientific research, it is studied as a model compound for nuclear receptor biology and as a reference standard in analytical work. This compound is offered as a freeze-dried powder of 10 mg per vial, exclusively for laboratory and research purposes.

What is SLU-PP-332?

SLU-PP-332 (4-hydroxy-N'-[(E)-naphthalene-2-ylmethylidene]benzohydrazide) is a small organic molecule developed at Saint Louis University, from which it derives its development code; in the literature, it also appears as SR9861. The structure couples a 4-hydroxybenzoyl unit to a naphthalene ring via a hydrazone bridge (C=N–N): a planar, highly conjugated structure with a phenol group at one end and an extensive aromatic system at the other. This combination makes the compound a useful model for studying ligand recognition by nuclear receptors.

In research models, SLU-PP-332 acts as an agonist of the three estrogen-related receptors ERRα, ERRβ, and ERRγ (nuclear receptors NR3B1–NR3B3). These are orphan receptors: structurally related to the estrogen receptor, but they do not bind estrogens themselves and are constitutively active in cell models in conjunction with co-activators from the PGC-1 family. In in vitro reporter assays, the compound induces transcription via all three subtypes, most strongly via ERRα (reported EC₅₀-values ​​of approximately 98 to 430 nM). The research focuses on the interaction with the ligand-binding domain and the subsequent expression of target genes. Consequently, SLU-PP-332 is used as a model compound and reference substance within scientific research.

Research areas

In a laboratory context, SLU-PP-332 occurs in the following research areas, among others:

• Receptor research into the ligand binding and transcriptional activation of ERRα, ERRβ, and ERRγ in isolated cell models in vitro.
• Molecular biological research into the interaction between ERR receptors and co-activators from the PGC-1 family in reporter assays.
• Structure-activity studies on acylhydrazones, varying substitutions in the phenol and naphthalene groups.
• Model studies of the transcription of mitochondrial target genes in cultured cell lines.
• Analytical method development and validation (HPLC, LC-MS) using SLU-PP-332 as the reference standard.
• Stability and solubility studies of hydrazone-containing molecules, including the sensitivity of the C=N bond to light and hydrolysis.

Each of these applications involves controlled laboratory or preclinical model research.

SLU-PP-332 in our research range

SLU-PP-332 falls under the category of freeze-dried research compounds in vial form within our product range. Researchers in this category frequently combine the compound with other lyophilized substances that are studied via their own biochemical targets. While SLU-PP-332 focuses on nuclear receptors and the resulting transcriptional regulation, other components within the same product line cover membrane-bound receptors, enzymes, or intracellular signaling pathways. These compounds share the freeze-dried format and vial packaging but differ fundamentally in molecular class and investigated mechanism.

Specifications

Shape, handling and storage

SLU-PP-332 is supplied as a freeze-dried powder with a standardized quantity of 10 mg per vial. Unlike solid tablet formulations, a lyophilisate is preceded by analytical or cell-based work in a suitable solvent; this description does not contain a protocol for this.

Store the vial refrigerated or at −20 °C, protected from direct light and moisture, in the original packaging; the hydrazone bond is sensitive to light and moisture. Handle the compound with standard laboratory precautions: work in a clean environment, use appropriate personal protective equipment, and prevent cross-contamination with other research materials. Keep the packaging out of reach of unauthorized persons.

Composition and lab results

This compound has a controlled purity of ≥ 99% (HPLC). The vial contains the freeze-dried molecule without added active ingredients.

You can find an overview of the available analytical documentation on our lab results pageThe reports are collected centrally there, so that you can incorporate the data into your own research records.

Frequently Asked Questions

To which dust class does SLU-PP-332 belong?
SLU-PP-332 is a small synthetic molecule from the class of acylhydrazones. It is not a peptide and therefore contains no amino acid sequence.

What is meant by a pan-ERR agonist?
In research models, the compound activates not one but all three estrogen-related receptors — ERRα, ERRβ, and ERRγ — albeit with different potency. This makes SLU-PP-332 a widely used model compound in mechanistic nuclear receptor research in vitro.

In what form is the compound supplied?
As a freeze-dried powder of 10 mg in a sealed vial, with a controlled purity of ≥ 99% (HPLC).

How do I store the vial?
Refrigerated or at −20 °C, protected from light and moisture, preferably in the original packaging.