P21 Nasal Spray Kit

P21 is a synthetic peptidomimetic from the group of neurotrophin-related model compounds, chemically an adamantyl-modified tetrapeptide amide. In scientific research, it is studied as a model compound for CNTF-related signal transduction and as a reference standard in analytical work. This compound is offered as a 10 mg freeze-dried lyophilisate in a nasal spray kit, exclusively for laboratory and research purposes.

What is P21?

P21 (N-acetyl-L-α-aspartylglycylglycyl-N-(3-carbamoyladamantan-1-yl)-L-leucinamide, designated in the literature by the development code P021) is a small synthetic peptide derivative derived from a four-amino acid fragment of the biologically active region of ciliary neurotrophic factor (CNTF). The Ac-Asp-Gly-Gly-Leu chain features an adamantane building block and an amide termination at the terminal. This combination of a very short peptide chain with a rigid, highly lipophilic hydrocarbon cage motif makes the compound a widely used model in peptidomimetic research: the adamantane unit increases lipophilicity and sterically shields the terminal against degradation by exopeptidases.

In research models, P21 is studied as a mimetic of that CNTF fragment. In cell models, the work focuses on the interaction with the CNTF receptor complex and the associated signaling cascades, including the expression of brain-derived neurotrophic factor (BDNF) and the associated TrkB-mediated pathways. In vitro, the compound serves as a reference ligand against which neurotrophin-dependent cell differentiation in culture systems is compared. Consequently, within scientific research, P21 is used as a model compound and reference substance.

Research areas

In a laboratory context, P21 occurs in the following research areas, among others:

• Receptor research into the CNTF receptor complex and gp130-coupled signal transduction in isolated cell models in vitro.
• Mechanistic study of neurotrophin signaling, with BDNF expression and TrkB-mediated pathways as reading parameters in cell culture.
• Structure-activity relationship research on adamantyl-modified peptidomimetics and the influence of lipophilic cage motifs on conformation and binding affinity.
• Enzymological research into the resistance of acetylated and amidated peptide chains to exo- and endopeptidases.
• Analytical method development and validation (HPLC, LC-MS) using P21 as the reference standard for small peptide derivatives.
• Stability and solubility studies of freeze-dried peptidomimetics under controlled temperature and humidity conditions.

Each of these applications involves controlled laboratory or preclinical model research.

P21 in our research range

P21 falls under the category of nasal spray kits within our range: a freeze-dried investigational compound supplied as a composite set together with a sterile sodium chloride solution and an empty spray bottle. Researchers working with this category frequently combine the compound with other freeze-dried compounds studied via their own biochemical targets. While P21 focuses on neurotrophin-related receptor signaling, other components within the same product line have been specifically chosen to cover diverse enzymatic, receptor-linked, or structural targets. These compounds share the kit format and the freeze-dried starting form, but differ fundamentally in molecular class and investigated mechanism.

Specifications

Shape, handling and storage

P21 is supplied as freeze-dried lyophilisate with a standardized quantity of 10 mg per vial. The kit also contains a sterile sodium chloride solution and an empty spray bottle. Unlike a solid tablet form, this freeze-dried starting form is not immediately available in a liquid state: reconstitution in a suitable medium is a prerequisite before analytical or cell culture work is possible. The lyophilisate is hygroscopic and must therefore be handled in a dry environment.

Store the vial in a cool, dark place, protected from direct light and moisture, in the original packaging; for extended storage periods, storage at −20 °C is customary for freeze-dried peptide derivatives. Handle the compound with standard laboratory precautions: work in a clean environment, use appropriate personal protective equipment, and prevent cross-contamination with other research materials. Keep the packaging out of reach of unauthorized persons.

Composition and lab results

The lyophilisate in this kit is supplied with a controlled purity of ≥ 99% (HPLC), as is customary for freeze-dried peptide derivatives. The content per vial is 10 mg of active compound.

You can find an overview of the available analytical documentation on our lab results pageThe reports are collected centrally there, so that you can incorporate the data into your own research records.

Frequently Asked Questions

To which chemical class does P21 belong?
P21 is a synthetic peptidomimetic: an acetylated and amidated tetrapeptide derivative whose end has been modified with an adamantane building block. It is therefore not a naturally occurring peptide, but a structural analogue of a CNTF fragment.

What role does the adamantane group play in research?
The adamantane unit is a rigid, highly lipophilic hydrocarbon cage. Research models study how this group influences the lipophilicity and conformation of the peptide fragment and how it sterically shields the molecule against enzymatic degradation by peptidases.

In what form is the compound supplied?
As freeze-dried lyophilisate of 10 mg per vial, assembled into a kit with a sterile sodium chloride solution and an empty spray bottle. The freeze-dried form requires reconstitution in a suitable medium.

How do I store the lyophilized powder?
Cool and dark, protected from light and moisture, preferably in the original packaging. For longer storage periods, freeze-dried material is generally stored at -20 °C.