Dutasteride 1 mg

Dutasteride is a synthetic small molecule of the class of 4-azasteroids, chemically an N-arylcarboxamide with a steroid-like ring skeleton. In scientific research, it is studied as a model compound for enzyme kinetics of steroid 5α-reductases and as a reference standard in analytical work. This compound is offered as tablets containing 1 mg of active substance per unit, exclusively for laboratory and research purposes.

What is Dutasteride?

Dutasteride, systematic (1S,3aS,3bS,5aR,9aR,9bS,11aS)-N-[2,5-bis(trifluoromethyl)phenyl]-9a,11a-dimethyl-7-oxo-dodecahydroindeno[5,4-f]quinoline-1-carboxamide, is a fully synthetic 4-azasteroid. The four-cyclic backbone follows the steroid geometry, but with a nitrogen atom at position 4 and a carboxamide function on the D-ring. Attached to this amide group is a phenyl ring with two trifluoromethyl substituents, a highly electron-withdrawing and lipophilic motif. This combination of a rigid, stereochemically fully fixed ring system and a fluorinated aryl group makes the compound a widely used model in the study of enzyme-ligand interaction in steroid-converting oxidoreductases.

In research models, dutasteride targets steroid-5α-reductase, an NADPH-dependent enzyme that breaks the double bond in 3-oxo-Δ⁴-steroids; the conversion of testosterone to 5α-dihydrotestosterone is the classic in vitro example of this. In enzyme kinetic systems, the compound acts as a slow-binding, virtually irreversible inhibitor that targets both the type 1 and type 2 isozyme, whereby a stable enzyme NADP⁺-adduct is formed. This behavior makes it particularly suitable for mechanistic enzymatic work on isozyme selectivity. Consequently, Dutasteride is used as a model compound and reference substance within scientific research.

Research areas

In a laboratory context, Dutasteride occurs in the following research areas, among others:

• Enzyme kinetic in vitro research into slow binding inhibition of steroid-5α-reductase and the formation of enzyme cofactor adducts.
• Isozyme selectivity analysis in which the type 1 and type 2 enzymes are characterized side-by-side in separate systems.
• Structure-activity study of 4-azasteroids and the influence of fluorinated aryl substituents on binding affinity.
• Model studies in cell culture systems on the enzymatic conversion of 3-oxo-Δ⁴-steroids under controlled conditions.
• Analytical method development and validation (HPLC, LC-MS, ¹⁹F-NMR) where Dutasteride serves as the reference standard.
• Stability and solubility studies of highly lipophilic, fluorinated small molecules under various analytical conditions.

Each of these applications involves controlled laboratory or preclinical model research.

Dutasteride in our research assortment

Dutasteride falls under the category of oral investigational compounds in solid tablet form within our range. While this compound targets the inhibition of an NADPH-dependent steroid reductase, other components within the same product line have been specifically chosen to cover atypical enzymatic or receptor-mediated targets. These compounds share the solid tablet form and the standardized amount of active substance per unit, but differ fundamentally in molecular class and investigated mechanism.

Specifications

Shape, handling and storage

Dutasteride is supplied as solid tablets containing a standardized amount of 1 mg of active substance per unit. Unlike freeze-dried peptides, this is a stable solid form: the tablets can be weighed directly in a laboratory setup for further analysis. Due to the high lipophilicity of the molecule, solubility in aqueous media is limited, which is a factor in the choice of method.

Store the tablets dry at room temperature, protected from direct light and moisture, in the original packaging. Observe the compound with the usual laboratory precautions: a clean working environment, suitable protective equipment, and no cross-contamination with other research material. Keep the packaging out of reach of unauthorized persons.

Composition and lab results

For this compound, the content is expressed as the amount of active substance per tablet (1 mg per unit) rather than as a purity percentage, as is customary for tablet and capsule formulations. The content is determined by chromatographic assay.

You can find an overview of the available analytical documentation on our lab results pageThe available reports are centrally collected there.

Frequently Asked Questions

To which class of substances does Dutasteride belong?
It is a synthetic small molecule from the group of 4-azasteroids: a steroid-like ring skeleton with a nitrogen atom in the A-ring and an N-arylcarboxamide on the D-ring. It is not a peptide and therefore contains no amino acid sequence.

What makes enzyme inhibition mechanistically interesting?
In research models, the compound binds slowly and almost irreversibly to steroid-5α-reductase, forming a stable complex with the cofactor NADP.⁺ arises. Because both the type 1 and type 2 isozyme are targeted, the compound is a useful reference point in comparative isozyme research.

In what form is the compound supplied?
As solid tablets with 1 mg of active substance per unit.

How do I store the tablets?
Dry, at room temperature and protected from light and moisture, preferably in the original packaging.