5-Amino-1MQ vial 50 mg

5-Amino-1MQ is a synthetic small molecule from the class of quaternary quinolinium salts, chemically a 5-amino-substituted 1-methylquinolinium. In scientific research, it is studied as a model compound for methyltransferase enzymology and as a reference standard in analytical work. This compound is offered as a freeze-dried powder of 50 mg per vial, exclusively for laboratory and research purposes.

What is 5-Amino-1MQ?

5-Amino-1MQ (1-methylquinolin-1-ium-5-amine, supplied as an iodide salt) is a small organic molecule surrounding a bicyclic quinolin skeleton. The ring nitrogen atom bears a methyl group, giving the molecule a permanently positively charged quaternary quinolinium core; an amino group is located at position 5. This fixed charge determines its behavior in the laboratory: unlike an ionizable amine, the charge does not change with pH, ​​making the molecule easy to handle in cation exchange chromatography. Additionally, the electron-rich amino substituent shifts the absorption band of the conjugated ring system and yields a distinct chromophore in the UV region. This makes the compound a widely used model molecule in the study of S-adenosylmethionine-dependent methylation reactions.

In research models, 5-Amino-1MQ is used to study the enzyme nicotinamide-N-methyltransferase (NNMT). NNMT transfers a methyl group from the cofactor S-adenosylmethionine (SAM) to nicotinamide, resulting in the formation of 1-methylnicotinamide and S-adenosylhomocysteine. In vitro, 5-Amino-1MQ binds to the substrate pocket of this enzyme and is therefore described in the literature as an inhibitor of this methylation step; the charged quinolinium motif mimics the methylated pyridinium structure of the reaction product. Subsequently, the consequences for nicotinamide and NAD are investigated in cell models.⁺-recycling route and for the methyl donor pool are followed. Within scientific research, 5-Amino-1MQ is therefore used as a model compound and reference substance.

Research areas

In a laboratory context, 5-Amino-1MQ occurs in the following research areas, among others:

• Enzymological investigation of nicotinamide-N-methyltransferase (NNMT) and the kinetics of SAM-dependent methyl transfer in vitro.
• Model studies on nicotinamide and NAD⁺-recycling pathway in cell models and cell-free enzyme systems.
• Structure-activity relationship study of quinolinium and isoquinolinium analogs as methyltransferase inhibitors.
• Biophysical binding studies of the interaction between the charged quinolinium motif and the substrate pocket.
• Analytical method development and validation (HPLC with UV detection, LC-MS) with 5-Amino-1MQ as the reference standard.
• Stability and solubility studies of quaternary ammonium salts under controlled conditions.

Each of these applications involves controlled laboratory or preclinical model research.

5-Amino-1MQ in our research assortment

5-Amino-1MQ falls within our range under the freeze-dried investigational compounds in vial form, a category that researchers regularly combine with substances being studied via their own biochemical target. While 5-Amino-1MQ focuses on a methyltransferase and its associated cofactor homeostasis, other components within the same product line have been specifically chosen to cover receptor-linked, metal-coordinated, or peptide-mediated targets. These compounds share the freeze-dried vial form but differ fundamentally in molecular class and investigated mechanism.

Specifications

Shape, handling and storage

5-Amino-1MQ is supplied as a freeze-dried powder of 50 mg per vial, sealed under a rubber stopper with aluminium sealing. It is a quaternary ammonium salt in solid form; the material is weighed in a laboratory setup and further processed according to the applicable analytical or enzymological protocol. Hygroscopic behaviour is a known point of attention for this class of substances.

Store the closed vial in a cool, dark place, protected from light and moisture, preferably in the original packaging. Iodide salts are sensitive to prolonged exposure to light, which may cause a change in the powder's color. Handle the compound with standard laboratory precautions: work in a clean environment, use appropriate personal protective equipment, and prevent cross-contamination. Keep the packaging out of reach of unauthorized persons.

Composition and lab results

The lyophilisate contains 50 mg of 5-Amino-1MQ per vial with a controlled purity of ≥ 99% (HPLC). No stabilizers or fillers are added, so that the compound remains usable as a reference material in analytical work.

You can find an overview of the available analytical documentation on our lab results page, where the reports are centrally collected for your own research records.

Frequently Asked Questions

To which substance class does 5-Amino-1MQ belong?
A small synthetic molecule: a quaternary quinolinium salt with an amino group at position 5, supplied as an iodide salt. It is not a peptide and therefore contains no amino acid sequence.

Which enzyme is central to the investigated mechanism?
Nicotinamide-N-methyltransferase (NNMT). In vitro, this enzyme transfers a methyl group from S-adenosylmethionine to nicotinamide; 5-Amino-1MQ is described in the literature as an inhibitor of this step.

In what form is the compound supplied?
As a freeze-dried powder of 50 mg in a sealed vial, without added excipients.

How do I store the vial?
Cool and dark, protected from light and moisture, preferably unopened in the original packaging.