FOXO4-DRI 10 mg

FOXO4-DRI is a synthetic research peptide, chemically a D-retro-inverso peptide derived from the p53-binding region of the transcription factor FOXO4. Within scientific research, it is studied as a model compound for protein-protein interactions and as a reference standard in analytical work. This compound is offered as a freeze-dried powder of 10 mg per vial, exclusively for laboratory and research purposes.

What is FOXO4-DRI?

FOXO4-DRI, also designated by the development code Proxofim, is a synthetic peptide with the molecular formula C₂₂₈H₃₈₈N₈₆O₆₄ and a molecular weight of approximately 5,358 Da. The compound is derived from that part of the transcription factor FOXO4 (Forkhead box O4) that provides binding to the protein p53. Characteristic is the D-retro-inverso structure: the amino acid sequence is reversed and all amino acids have been replaced by their mirror-image D-form. As a result, the spatial arrangement of the side chains remains comparable to that of the original L-peptide, while the chain is much less susceptible to degradation by proteases. This combination makes FOXO4-DRI a widely used model in the study of peptidomimetics and stabilized inhibitors of protein-protein interactions.

In research models, FOXO4-DRI is described as an antagonist of the interaction between FOXO4 and p53. In cell models in which cellular senescence has been induced, these two proteins form complexes in the cell nucleus; in vitro, the peptide disrupts this complex formation, after which p53 distributes differently throughout the cell and the intrinsic mitochondrial apoptosis pathway is initiated. Because this is observed selectively in senescent cells in culture systems, rather than in normally dividing cells, the compound is a useful tool for analyzing this selectivity. Consequently, FOXO4-DRI is used as a model compound and reference substance within scientific research.

Domenii de cercetare

In a laboratory context, FOXO4-DRI occurs in the following research areas, among others:

• Research into the protein-protein interaction between FOXO4 and p53 and its disruption in isolated cell models in vitro.
• Mechanistic cell biological research into the intrinsic, mitochondrially mediated apoptosis pathway in culture systems.
• Biophysical model studies of D-retro-inverso peptides and the structure-activity relationship between mirror-image and natural amino acid sequences.
• Model studies of subcellular localization of transcription factors and their binding partners in cell culture systems.
• Analytical method development and validation (HPLC, LC-MS) using FOXO4-DRI as the reference standard.
• Stability and solubility studies of protease-resistant peptides under controlled conditions.

Fiecare dintre aceste aplicații implică cercetare controlată de laborator sau pe modele preclinice.

FOXO4-DRI in our research range

FOXO4-DRI falls under the category of freeze-dried research peptides in vial form within our range. Researchers working with this category frequently combine the compound with other peptides studied via their own biochemical target. While FOXO4-DRI focuses on disrupting a specific protein-protein interaction, other components within the same product line have been chosen specifically to cover receptor-bound or enzymatic targets. These compounds share the freeze-dried vial form but differ fundamentally in molecular structure and investigated mechanism.

Specificații

Formă, manipulare și depozitare

FOXO4-DRI is supplied as a freeze-dried powder of 10 mg per vial. This lyophilisate is placed in a suitable solvent in a laboratory setup prior to analysis.

A se păstra flaconul la frigider, ferit de lumina directă și umiditate, în ambalajul original. Manipulați compusul respectând precauțiile standard de laborator: lucrați într-un mediu curat, utilizați echipament individual de protecție adecvat și preveniți contaminarea încrucișată cu alte materiale de cercetare. A nu se lăsa la îndemâna persoanelor neautorizate.

Compoziție și rezultate de laborator

A controlled purity of ≥ 99% (HPLC) is maintained for this lyophilisate. The vial contains the peptide as a dry substance without added excipients.

Puteți găsi o prezentare generală a documentației analitice disponibile pe site-ul nostru pagina cu rezultate de laboratorRapoartele sunt colectate centralizat acolo, astfel încât să puteți încorpora datele în propriile înregistrări de cercetare.

Întrebări frecvente

To which substance class does FOXO4-DRI belong?
FOXO4-DRI is a synthetic peptide composed of D-amino acids in reverse order, a construction referred to in the literature as D-retro-inverso. It is therefore a peptide with a relatively high molecular weight of approximately 5,358 Da.

What exactly does the D-retro-inverso structure entail?
The amino acid sequence of the original peptide is reversed, and each amino acid is replaced by its mirror-image D variant. This double reversal yields a chain in which the side chains remain spatially similarly oriented, while the peptide bonds are much more difficult for proteases to recognize.

Sub ce formă este furnizat compusul?
As freeze-dried powder of 10 mg in a sealed vial, one vial per package.

Cum depozitez fiola?
Refrigerated, protected from light and moisture, and preferably in the original packaging. Limit the number of temperature changes.