Soluție salină 5 ml (NaCl 0,9%)

0.9% saline solution is an inorganic electrolyte solution, chemically an aqueous solution of sodium chloride in isotonic concentration. In scientific research, it is used as a diluent, as a blank matrix, and as a reference medium with a fixed ionic strength in analytical and cell-related work. This solution is offered as an individually sealed 5 ml ampoule without preservative, exclusively for laboratory and research purposes.

What is Saline Solution (NaCl 0.9%)?

0.9% saline solution, also referred to as physiological or isotonic sodium chloride solution, consists of 0.9% (m/v) sodium chloride in purified water. Sodium chloride dissociates completely into sodium and chloride ions in water; consequently, the solution is not a molecular mixture but an electrolyte with an accurately calculable ionic strength. At this concentration, the theoretical osmolarity is approximately 308 mOsm/l, which makes the solution isotonic with respect to most cell-based research models: cells or tissue preparations retain their volume in this medium. This combination of a fully defined composition and a physiologically relevant ionic strength makes the solution the standard medium in laboratory work.

Because the solution contains no buffering system and no preservative, it is chemically as non-interfering as an aqueous matrix can be. There are no organic excipients present that could interfere with chromatographic separation or cause background signaling. At the same time, the dissolved ions co-determine the behavior of biomolecules: ion strength influences solubility, charge shielding, and aggregation tendency, making the solution useful in physico-chemical research. Consequently, 0.9% saline solution is used as a reference medium and diluent in scientific research.

Domenii de cercetare

In a laboratory context, 0.9% saline solution is found in the following research areas, among others:

• Dilution series from concentrated stock solutions, in which the ionic strength remains constant across the series.
• Analytical method development and validation, with the solution as a blank matrix for background and matrix effects.
• Physical-chemical research into the effect of ionic strength on the solubility and aggregation of biomolecules.
• Cell culture and tissue models in which an isotonic, non-buffering medium is required to rinse preparations without osmotic loading.
• Verification and calibration of measurement setups for conductivity, osmolarity, and refractive index, with the known concentration as the reference point.
• Compatibility and materials testing: rinsing glassware and assessing the interaction between an aqueous salt solution and packaging material.

Fiecare dintre aceste aplicații implică cercetare controlată de laborator sau pe modele preclinice.

Saline solution in our research range

0.9% saline solution falls under laboratory supplies in our range and not under the research compounds themselves: it is not a study object but an aid in the setup. Researchers combine the solution with other consumables and with liquids that have their own function, for example because they do contain a buffering system. These items share a supporting role but differ in composition and in the extent to which they can influence a measurement.

Specificații

Formă, manipulare și depozitare

The solution is supplied in individually sealed 5 ml ampoules. Each ampoule is intended for single use: because it contains no preservative, there is nothing present to inhibit microbial growth in an opened ampoule, and an opened ampoule is therefore not suitable for subsequent use. It is a ready-to-use liquid that requires no further processing.

Store the unopened ampoules in a cool, dry place in the original packaging, protected from direct light, and check the liquid for clarity and visible particles before use. Handle the material with standard laboratory precautions: work in a clean environment, use appropriate personal protective equipment, and prevent cross-contamination with other research materials. Keep the packaging out of reach of unauthorized persons.

Compoziție și rezultate de laborator

The composition is not expressed as a purity percentage, but as the concentration of the dissolved salt: 0.9% (m/v) sodium chloride in purified water, without preservatives, buffer salts, or other additives. The relevant specifications are therefore the concentration, osmolarity, acidity, and clarity.

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Întrebări frecvente

To which dust class does this solution belong?
It is an inorganic electrolyte solution: sodium chloride, completely dissociated in water. It contains no organic compound, no peptide, and no preservative.

Why is an isotonic solution important in research?
At approximately 308 mOsm/l, the osmotic pressure difference is practically zero with most cell-bound model systems, so that preparations neither absorb nor lose water. In addition, a fixed ion strength keeps the conditions constant over a dilution series.

In what form is the solution delivered?
As a clear, colourless liquid in an individually sealed 5 ml ampoule, intended for single use.

How do I store the ampoules?
Store in a cool, dry place, protected from direct light and in the original packaging. Do not store an opened ampoule, as the solution contains no preservative.