Alprostadil 20 mcg

Alprostadil is an eicosanoid, chemically the synthetic form of prostaglandin E.₁: a C₂₀-carboxylic acid with a substituted cyclopentanone ring. Within scientific research, it is studied as a model compound for prostanoid receptors and eicosanoid chemistry and as a reference standard in analytical work. This compound is offered as a freeze-dried powder of 20 mcg per sealed vial, exclusively for laboratory and research purposes.

What is Alprostadil?

Alprostadil, systematic (13E,15S)-11α,15-dihydroxy-9-oxoprost-13-enoic acid, is composed of a five-membered ring with a ketone group at position 9 and two long side chains: a saturated chain with a terminal carboxylic acid group and a chain with one trans-double bond and a hydroxyl group in the S-configuration at position 15. The compound is distinct from prostaglandin E₂ due to the absence of the double bond between C5 and C6: one structural difference, making the molecule suitable for investigating the influence of side-chain unsaturation on receptor recognition. Chemically, it is a labile molecule: the combination of a β-hydroxyketone in the ring and an allylic hydroxyl group makes the structure susceptible to water loss and isomerization, successively producing prostaglandin A₁ and the conjugated prostaglandin B₁ arise. Consequently, the compound serves as a classic model in stability studies of prostanoids.

In research models, prostaglandin E intervenes₁ It attaches to the EP receptor family: four G-protein-coupled subtypes (EP1 to EP4) that activate diverse pathways, including cAMP enhancement via Gs and calcium mobilization via Gq. This makes the compound a common reference ligand for mapping subtype selectivity. Enzymatically, the 15-hydroxyl group is oxidized to a keto group by 15-hydroxyprostaglandin dehydrogenase, which significantly reduces affinity; this rapid conversion makes the substance attractive for enzymological research. Consequently, Alprostadil is used as a model compound and reference substance in scientific research.

Aree di ricerca

In a laboratory context, Alprostadil occurs in the following research areas, among others:

• Binding studies of the EP1 to EP4 subtypes in cell lines and membrane preparations in vitro.
• Research into signal transduction: cAMP formation and calcium mobilization after receptor activation in cell models.
• Enzymological investigation of 15-hydroxyprostaglandin dehydrogenase and the oxidation of the 15-hydroxyl group.
• Model research into vascular tone in isolated tissue preparations, with the compound as a comparison standard.
• Structure-activity research within the prostanoids, focusing on the stereochemistry at positions 11 and 15.
• Stability studies regarding water separation and isomerization, and analytical method development using LC-MS/MS, are necessary because the molecule itself exhibits hardly any UV absorption.

Ciascuna di queste applicazioni prevede ricerche controllate in laboratorio o su modelli preclinici.

Alprostadil in our research assortment

Alprostadil falls under the category of freeze-dried research compounds in sealed vials within our product range. While Alprostadil is a lipid signaling molecule that targets prostanoid receptors, other components within the same product line focus on peptide chains or enzymatic targets. They share the freeze-dried form and associated storage requirements, but differ fundamentally in molecular class and investigated mechanism.

Specifiche

Forma, manipolazione e stoccaggio

Alprostadil is supplied as a freeze-dried powder in a sealed vial with 20 mcg per unit. The lyophilisate is a solid, fine mass that is only dissolved in the laboratory setup, according to the laboratory's own procedure; in analytical work, prostaglandins are usually dissolved in ethanol or a similar organic medium, because aqueous solutions accelerate chemical degradation.

Store the unopened vial in a cool, dry, and dark place in the original packaging; the structure is sensitive to heat, moisture, and non-neutral acidity. Handle the material with standard laboratory precautions: work in a clean environment, use appropriate personal protective equipment, and prevent cross-contamination with other research materials. Keep the packaging out of reach of unauthorized persons.

Composizione e risultati di laboratorio

The material in the vial is the freeze-dried compound with a controlled purity of ≥ 99% (HPLC). For this class of substances, chromatographic separation also reveals the dehydration and isomerization products that arise during improper storage.

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Domande frequenti

To which class of substances does Alprostadil belong?
Alprostadil is an eicosanoid: a lipid signaling molecule with twenty carbon atoms from the prostaglandin family. It is not a peptide and therefore contains no amino acid sequence.

What happens to the 15-hydroxyl group in research models?
That group is oxidized to a keto group by 15-hydroxyprostaglandin dehydrogenase. The resulting 15-keto product binds much more weakly to the EP receptors, making this step central to mechanistic research on prostanoids.

In quale forma viene fornito il composto?
As freeze-dried powder in a sealed vial, with 20 mcg per vial.

Come devo conservare la fiala?
Cool, dry, and dark, in the original packaging. The freeze-dried powder is significantly more stable than a solution; solutions are stored refrigerated and preferably used shortly after preparation.