GHK-Cu and SNAP-8 Serum 35 ml

GHK-Cu and SNAP-8 Serum is a two-component reference formulation of synthetic peptides, chemically a combination of a copper(II) tripeptide complex and an acetylated, C-terminally amidated octapeptide. Within scientific research, the formulation is studied as a multicomponent model system for coordination chemistry and peptide stability, and as a reference mixture in analytical work. This compound is offered as a 35 ml aqueous formulation, exclusively for laboratory and research purposes.

What is the GHK-Cu and SNAP-8 Serum?

The first component is glycyl-L-histidyl-L-lysine, coordinated to a copper(II) ion. The tripeptide binds the metal ion via the imidazole nitrogen atom of the histidine residue, a deprotonated amide nitrogen from the main chain, and the N-terminal amino group, resulting in a tetragonally distorted square-plane coordination pattern. This geometry gives the complex a dd absorption band around 500–600 nm and makes it highly spectroscopically traceable. The second component, SNAP-8, is an octapeptide with an acetylated N-terminus and an amidated C-terminus; both modifications neutralize the charges at the chain ends, reducing the net charge and measurably altering the retention behavior on inverted-phase columns.

In research models, the components are studied for different points of action: the copper complex as a model for copper-dependent processes and for the expression of matrix components in fibroblast cell models, and SNAP-8 as a structural analogue of the N-terminal segment of the SNAP-25 protein in cell-free research into SNARE complex formation. Because both substances are present in the same matrix, the formulation simultaneously constitutes a multicomponent reference mixture: a method that displays both peaks completely separated demonstrates that the resolving power is sufficient. Consequently, within scientific research, this formulation is used as both a model compound and a reference substance.

Research areas

In a laboratory context, GHK-Cu and SNAP-8 Serum occur in the following research areas, among others:

• Coordination chemistry studies of the binding geometry of copper(II) to histidine-containing tripeptides.
• Spectroscopic method development with the copper complex as the detection and calibration reference in the visible region.
• Structure-activity study into the effect of N-terminal acetylation and C-terminal amidation on charge distribution and conformation.
• Research into SNARE complex assembly and competitive peptide binding in cell-free model systems.
• Analytical method development and validation (HPLC, LC-MS) with the formulation as a multicomponent reference mixture.
• Stability and solubility studies of peptides and metal complexes in aqueous matrix.

Each of these applications involves controlled laboratory or preclinical model research.

The GHK-Cu and SNAP-8 Serum in our research range

Within our range, this formulation falls under the topical research formulations: aqueous matrices in which the active ingredients are supplied already dissolved. While this formulation combines two active ingredients with different points of action, other components within the same line contain either a single defined active ingredient or an active ingredient from a different molecular class. They share the liquid form but differ in composition and the mechanism under investigation.

Specifications

Shape, handling and storage

The formulation is supplied as a clear to light blue colored liquid in a 35 ml vial; the color results from ligand field absorption of the copper(II) center. Both active ingredients are already dissolved, so the formulation can be used as a liquid sample without pretreatment. Exact concentrations are provided upon request.

Store the vial cool at 2–8 °C, protected from light and heat, and close it after each sampling; aqueous peptide matrices are sensitive to temperature fluctuations and microbial contamination. Handle the formulation with customary laboratory precautions: clean environment, suitable personal protective equipment, sampling with clean equipment, and out of reach of unauthorized persons.

Composition and lab results

The raw materials in this formulation are research-grade and are offered with a controlled purity of ≥ 99% (HPLC). For the copper complex, the stoichiometry between the tripeptide and the metal ion determines the spectroscopic profile.

You can find an overview of the available analytical documentation on our lab results page, where the reports are centrally collected.

Frequently Asked Questions

To which class of substances do the two components belong?
The first is a metal-peptide complex: a tripeptide that coordinates a copper(II) ion. The second is a synthetic octapeptide with an acetylated N-terminus and an amidated C-terminus.

Why is coordination geometry relevant?
The surround of the copper(II) ion determines the energy of the dd junctions and thereby the absorption band around 500–600 nm; that band is a direct indicator of the integrity of the coordination.

In what form is the formulation supplied?
As an aqueous formulation of 35 ml with preservative, in which both active ingredients are already present in dissolved form.

How do I save the wording?
Keep cool at 2–8 °C, protected from direct light and heat, with the bottle tightly closed.