Ara-290 vial 10 mg

Ara-290 is a synthetic research peptide, chemically a linear undecapeptide derived from the B-helix domain of erythropoietin. Within scientific research, it is studied as a model compound for receptor and cytokine signaling and as a reference standard in analytical work. This compound is offered as a freeze-dried powder of 10 mg per vial, exclusively for laboratory and research purposes.

What is Ara-290?

Ara-290 (L-pyroglutamyl-L-glutamyl-L-glutaminyl-L-leucyl-L-glutamyl-L-arginyl-L-alanyl-L-leucyl-L-asparaginyl-L-seryl-L-serine) is an eleven-amino acid linear peptide with the sequence Pyr-Glu-Gln-Leu-Glu-Arg-Ala-Leu-Asn-Ser-Ser. The chain is modeled after the outer surface of the β-helix of erythropoietin, which has earned the compound the designation pyroglutamate-helix-β-surface-peptide (pHBSP) in the literature. The N-terminal pyroglutamate terminates the amino group of the chain, a structural feature that makes the peptide less susceptible to degradation by aminopeptidases in vitro. This makes Ara-290 a useful model in the study of peptide receptor recognition.

In research models, Ara-290 does not bind to the classical homodimeric erythropoietin receptor, but selectively to a heteromeric receptor complex composed of the EPO receptor (EPOR) and the common beta chain CD131 (βcR). This complex is referred to in the scientific literature as the innate repair receptor (IRR). Consequently, in cell models, the peptide can be used to study both signaling pathways separately. Within scientific research, Ara-290 is therefore used as a model compound and reference substance.

Research areas

In a laboratory context, Ara-290 occurs in the following research areas, among others:

• Receptor research into the EPOR/CD131 heteromere and its comparison with the homodimeric EPO receptor in cultured cell lines.
• Research into intracellular signal transduction (including JAK2/STAT and PI3K/Akt pathways) after receptor binding in vitro.
• Structure-activity relationship study in which individual amino acid positions of the undecapeptide are varied.
• Biophysical model studies of peptide fragments that mimic a helical surface of a larger protein.
• Analytical method development and validation (HPLC, LC-MS) using Ara-290 as the reference standard.
• Stability and solubility studies of short linear peptides with a blocked N-terminus under controlled conditions.

Each of these applications involves controlled laboratory or preclinical model research.

Ara-290 in our research range

Within our range, Ara-290 falls under the category of freeze-dried research peptides in vial form. Researchers regularly combine the compound with other peptides that are studied via their own biochemical target. While Ara-290 targets a heteromeric receptor complex from the cytokine receptor family, other components within the same product line have been selected to cover divergent receptor families or structural motifs. These compounds share the freeze-dried vial form but differ fundamentally in amino acid sequence, molecular size, and investigated mechanism.

Specifications

Shape, handling and storage

Ara-290 is supplied as a freeze-dried, white to off-white powder with a standardized quantity of 10 mg per vial. Unlike a solid tablet form, this is a lyophilisate: prior to analytical work, the material is brought into solution, depending on the laboratory's own protocol. The freeze-dried powder is hygroscopic and should be exposed to ambient air for the shortest possible time.

Store the unopened vial in a cool, dry place, protected from direct light, in the original packaging; for longer storage, a freezer at −20 °C is customary. Observe the compound with the usual laboratory precautions: work in a clean environment, use appropriate personal protective equipment, and prevent cross-contamination with other research materials. Keep the packaging out of reach of unauthorized persons.

Composition and lab results

For this lyophilisate, a controlled purity of ≥ 99% (HPLC) is maintained. The stated quantity of 10 mg refers to the peptide itself, without added active ingredients.

You can find an overview of the available analytical documentation on our lab results pageThe reports are collected centrally there, so that you can incorporate the data into your own research records.

Frequently Asked Questions

To which dust class does Ara-290 belong?
Ara-290 is a synthetic linear peptide of eleven amino acids (an undecapeptide) with the sequence Pyr-Glu-Gln-Leu-Glu-Arg-Ala-Leu-Asn-Ser-Ser. It is not a small organic molecule, but a peptide fragment that mimics the surface of the glycoprotein erythropoietin.

What is meant by the innate repair receptor in research?
In the literature, this refers to a heteromeric receptor complex of the EPO receptor (EPOR) and the common beta chain CD131. In cell models, Ara-290 targets this complex and not the homodimeric EPO receptor, which makes both signaling pathways distinguishable in vitro.

In what form is the connection supplied?
As a freeze-dried powder in a vial containing 10 mg peptide. The lyophilisate is dissolved prior to analytical use.

How do I store the vial?
Freeze-dried, cool, dry and protected from light in the original packaging; for longer storage at −20 °C.