NAD+ (nicotinamide-adenine-dinucleotide) is a naturally occurring coenzyme, chemically a dinucleotide composed of a nicotinamide and an adenine nucleotide. In scientific research, it is studied as a model compound for redox biochemistry and enzyme kinetics and as a reference standard in analytical work. This compound is offered as a 3 ml pre-filled injection pen containing 500 mg, exclusively for laboratory and research purposes.<\/p>\n
NAD+ (\u03b2-nicotinamide-adenine-dinucleotide) is a dinucleotide in which two ribonucleotides are joined via their 5\u2032-phosphate groups to form a pyrophosphate bridge: an adenine ribonucleotide on one side and a nicotinamide ribonucleotide with a positively charged pyridinium ring on the other. This asymmetric structure\u2014a recognizable adenosine region next to a redox-active pyridinium ring\u2014makes the compound a widely used model in the study of cofactor recognition, binding site geometry, and electron transfer.<\/p>\n
In research models, NAD+ functions as a reversible hydride acceptor: the nicotinamide ring accepts a hydride ion at position C4, resulting in the reduced form NADH. This NAD+\/NADH couple forms the basis of numerous in vitro enzyme assays, because the conversion can be monitored spectrophotometrically by the characteristic absorption of NADH at 340 nm. Additionally, NAD+ is studied in cell models as a co-substrate for NAD+-consuming enzymes such as sirtuins, poly(ADP-ribose) polymerases, and CD38, which cleave the glycosidic bond between nicotinamide and ADP-ribose, thereby releasing nicotinamide. Consequently, NAD+ is used as a model compound and reference substance within scientific research.<\/p>\n
In a laboratory context, NAD+ occurs in the following research areas, among others:<\/p>\n
Each of these applications involves controlled laboratory or preclinical model research.<\/p>\n
This version belongs to the line of pre-filled injection pens: compounds contained in dissolved form in a sealed cartridge, so that a researcher does not need to go through their own dissolution step. The same compound is available elsewhere in the range as freeze-dried lyophilisate in a vial. Other compounds within the pen line act on a completely different biochemical system; the form indicates nothing about the substance class.<\/p>\n
| Specification<\/th>\n | Value<\/th>\n<\/tr>\n<\/thead>\n | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Sample<\/td>\n | NAD+ (nicotinamide adenine dinucleotide)<\/td>\n<\/tr>\n | ||||||||||||
| Synonyms<\/td>\n | \u03b2-NAD; \u03b2-nicotinamide adenine dinucleotide; diphosphopyridine nucleotide (DPN); coenzyme I<\/td>\n<\/tr>\n | ||||||||||||
| CAS number<\/td>\n | 53-84-9<\/td>\n<\/tr>\n | ||||||||||||
| Molecular formula \/ weight<\/td>\n | C21<\/sub>H27<\/sub>N7<\/sub>O14<\/sub>P2<\/sub> \/ 663.43 Da<\/td>\n<\/tr>\n| PubChem CID<\/td>\n | 5892<\/td>\n<\/tr>\n | Class<\/td>\n | Dinucleotide coenzyme, redox cofactor (non-peptide)<\/td>\n<\/tr>\n | Form<\/td>\n | Pre-filled injection pen with sealed cartridge<\/td>\n<\/tr>\n | Quantity per unit<\/td>\n | 500 mg per 3 ml pen<\/td>\n<\/tr>\n | Quantity per package<\/td>\n | 1 pen, 10 pen needles and alcohol wipes<\/td>\n<\/tr>\n | Storage<\/td>\n | Refrigerated at 2\u20138 \u00b0C, store in a dark place.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n | Shape, handling and storage<\/h2>\nThe compound is dissolved in a sealed 3 ml glass cartridge in a pen with a click mechanism. A separate dissolution step is not required with this version; the cartridge remains closed until a needle is inserted. The label includes a table showing the relationship between click settings and dispensed volume, allowing sample collections to be reproducibly recorded in a protocol.<\/p>\n Store the pen refrigerated between 2 and 8 \u00b0C, protected from light and in the original packaging. Handle the pen in a clean working environment, use a new sterile needle for each draw to prevent cross-contamination, and wear standard personal protective equipment. Keep the material out of reach of unauthorized persons.<\/p>\n Composition and lab results<\/h2>\nThe raw material used has a controlled purity of \u2265 99% (HPLC). The pen contains NAD+ in a sterile aqueous base; the total amount of active substance is stated on the label for each version.<\/p>\n |