{"id":2905,"date":"2026-09-01T09:35:26","date_gmt":"2026-09-01T09:35:26","guid":{"rendered":"https:\/\/pevropeptides.com\/?post_type=product&#038;p=2905"},"modified":"2026-09-02T12:24:46","modified_gmt":"2026-09-02T12:24:46","slug":"kpv","status":"publish","type":"product","link":"https:\/\/pevropeptides.com\/cs\/product\/kpv\/","title":{"rendered":"KPV"},"content":{"rendered":"<h2>Co je KPV?<\/h2>\n<p>KPV je syntetick\u00fd tripeptid: \u0159et\u011bzec t\u0159\u00ed aminokyselinov\u00fdch zbytk\u016f se sekvenc\u00ed Lys-Pro-Val (lysin-prolin-valin). Slou\u010denina je zn\u00e1m\u00e1 pod \u010d\u00edslem CAS 67727-97-3 s molekul\u00e1rn\u00edm vzorcem C<sub>16<\/sub>H<sub>30<\/sub>N<sub>4<\/sub>O<sub>4<\/sub> (p\u0159ibli\u017en\u011b 342,44 Da) a PubChem CID 125672. Se t\u0159emi aminokyselinov\u00fdmi zbytky pat\u0159\u00ed KPV k nejkrat\u0161\u00edm mo\u017en\u00fdm oligopeptid\u016fm, a proto tvo\u0159\u00ed doln\u00ed limitn\u00ed p\u0159\u00edpad t\u0159\u00eddy peptid\u016f.<\/p>\n<p>Struktura je chemicky ost\u0159e definovan\u00e1: bazick\u00fd lysinov\u00fd postrann\u00ed \u0159et\u011bzec s volnou \u03b5-aminoskupinou na N-konci, centr\u00e1ln\u00ed prolin, kter\u00fd omezuje rota\u010dn\u00ed volnost kolem sousedn\u00ed peptidov\u00e9 vazby, a nepol\u00e1rn\u00ed valin na C-konci. V d\u016fsledku toho molekula nese jak kladn\u011b nabitou, tak nepol\u00e1rn\u00ed \u010d\u00e1st pouze na t\u0159ech pozic\u00edch. Pr\u00e1v\u011b tato kombinace kr\u00e1tk\u00e9 d\u00e9lky \u0159et\u011bzce, vysok\u00e9 polarity a cis\/trans-izomerizovateln\u00e9 vazby X-Pro \u010din\u00ed KPV mimo\u0159\u00e1dn\u011b vhodn\u00fdm pro v\u00fdvoj metod: kr\u00e1tk\u00e9 \u0159et\u011bzce nevykazuj\u00ed t\u00e9m\u011b\u0159 \u017e\u00e1dnou retenci na standardn\u00edch kolon\u00e1ch s reverzn\u00ed f\u00e1z\u00ed a vyplavuj\u00ed se bl\u00edzko mrtv\u00e9ho objemu, co\u017e z t\u00e9to slou\u010deniny \u010din\u00ed p\u0159\u00edsn\u00fd testovac\u00ed p\u0159\u00edpad pro v\u00fdb\u011br kolony, iontov\u011b p\u00e1rovac\u00ed \u010dinidla a podm\u00ednky HILIC. Skute\u010dnost, \u017ee KPV neobsahuje \u017e\u00e1dn\u00e9 aromatick\u00e9 zbytky, a proto t\u00e9m\u011b\u0159 neabsorbuje nad 220 nm, z n\u011bj \u010din\u00ed u\u017eite\u010dn\u00fd model pro detek\u010dn\u00ed probl\u00e9my zahrnuj\u00edc\u00ed slab\u011b UV aktivn\u00ed peptidy.<\/p>\n<h2>Aplikace v laboratorn\u00edm kontextu<\/h2>\n<p>V r\u00e1mci peptidov\u00e9 chemie a analytick\u00e9 pr\u00e1ce se KPV vyskytuje mimo jin\u00e9 v n\u00e1sleduj\u00edc\u00edch oblastech v\u00fdzkumu in vitro:<\/p>\n<ul>\n<li><strong>V\u00fdzkum vztahu struktura-aktivita<\/strong> ohledn\u011b samostatn\u00e9ho p\u0159\u00edsp\u011bvku bazick\u00fdch, rigidiza\u010dn\u00edch a nepol\u00e1rn\u00edch pozic k rozlo\u017een\u00ed n\u00e1boje, polarit\u011b a reten\u010dn\u00edmu chov\u00e1n\u00ed velmi kr\u00e1tk\u00fdch \u0159et\u011bzc\u016f.<\/li>\n<li><strong>V\u00fdzkum peptidov\u00e9 chemie<\/strong> sm\u011brem k rozpustnosti a konforma\u010dn\u00edmu chov\u00e1n\u00ed tripeptid\u016f, v\u010detn\u011b pom\u011bru cis\/trans kolem vazby Lys-Pro monitorovan\u00e9ho NMR spektroskopi\u00ed.<\/li>\n<li><strong>Studie enzymatick\u00e9 stability<\/strong> ve kter\u00e9m se ur\u010duje, jak \u0161patn\u011b jsou vazby X-Pro \u0161t\u011bpeny v\u011bt\u0161inou prote\u00e1z za definovan\u00fdch podm\u00ednek, p\u0159i\u010dem\u017e tripeptid slou\u017e\u00ed jako jednoduch\u00fd referen\u010dn\u00ed bod.<\/li>\n<li><strong>V\u00fdvoj a validace analytick\u00fdch metod<\/strong> (HPLC, LC-MS, mapov\u00e1n\u00ed peptid\u016f), ve kter\u00fdch jsou optimalizov\u00e1ny retence, tvar p\u00edku a detek\u010dn\u00ed limit n\u00edzkomolekul\u00e1rn\u00edch pol\u00e1rn\u00edch peptid\u016f, s derivatizac\u00ed nebo hmotnostn\u00ed spektrometri\u00ed jako alternativou k UV z\u00e1\u0159en\u00ed.<\/li>\n<\/ul>\n<h2>Specifikace<\/h2>\n<table>\n<tbody>\n<tr>\n<td><strong>Ochutnat<\/strong><\/td>\n<td>KPV (syntetick\u00fd tripeptid), lyofilizovan\u00fd v uzav\u0159en\u00e9 lahvi\u010dce<\/td>\n<\/tr>\n<tr>\n<td><strong>D\u00e9lka peptidu<\/strong><\/td>\n<td>3 aminokyselinov\u00e9 zbytky<\/td>\n<\/tr>\n<tr>\n<td><strong>Sekvence<\/strong><\/td>\n<td>Lys-Pro-Val (lysin-prolin-valin)<\/td>\n<\/tr>\n<tr>\n<td><strong>\u010c\u00edslo CAS<\/strong><\/td>\n<td>67727-97-3<\/td>\n<\/tr>\n<tr>\n<td><strong>Molekul\u00e1rn\u00ed vzorec \/ hmotnost<\/strong><\/td>\n<td>C<sub>16<\/sub>H<sub>30<\/sub>N<sub>4<\/sub>O<sub>4<\/sub> \/ cca 342,44 Da<\/td>\n<\/tr>\n<tr>\n<td><strong>T\u0159\u00edda<\/strong><\/td>\n<td>Syntetick\u00fd line\u00e1rn\u00ed tripeptid (kr\u00e1tk\u00fd model oligopeptidu)<\/td>\n<\/tr>\n<tr>\n<td><strong>Formul\u00e1\u0159<\/strong><\/td>\n<td>Lyofilizovan\u00fd pr\u00e1\u0161ek v uzav\u0159en\u00e9 sklen\u011bn\u00e9 lahvi\u010dce<\/td>\n<\/tr>\n<tr>\n<td><strong>\u010cistota<\/strong><\/td>\n<td>\u2265 99 % (HPLC)<\/td>\n<\/tr>\n<tr>\n<td><strong>Skladov\u00e1n\u00ed<\/strong><\/td>\n<td>Lyofilizov\u00e1no p\u0159i 2\u20138 \u00b0C, tmav\u00e9 a such\u00e9<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h2>Tvar, manipulace a skladov\u00e1n\u00ed<\/h2>\n<p>Materi\u00e1l se dod\u00e1v\u00e1 jako lyofilizovan\u00fd pr\u00e1\u0161ek (lyofilizovan\u00fd pr\u00e1\u0161ek), b\u00edl\u00e9 a\u017e t\u00e9m\u011b\u0159 b\u00edl\u00e9 barvy, v uzav\u0159en\u00e9 sklen\u011bn\u00e9 lahvi\u010dce s pry\u017eovou p\u0159ep\u00e1\u017ekou a hlin\u00edkov\u00fdm smr\u0161\u0165ovac\u00edm uz\u00e1v\u011brem. Kr\u00e1tk\u00e9, vysoce pol\u00e1rn\u00ed peptidy jsou v\u00fdrazn\u011b hygroskopick\u00e9, proto z\u016fst\u00e1v\u00e1 lahvi\u010dka uzav\u0159en\u00e1 v p\u016fvodn\u00edm obalu, dokud nen\u00ed vzorek zpracov\u00e1n v laborato\u0159i, proto\u017ee absorbovan\u00e1 vlhkost m\u00e1 p\u0159i stanoven\u00ed hmotnosti p\u0159i n\u00edzk\u00e9 molekulov\u00e9 hmotnosti relativn\u011b velk\u00fd vliv.<\/p>\n<p>Uzav\u0159enou lahvi\u010dku skladujte lyofilizovanou p\u0159i teplot\u011b 2\u20138 \u00b0C na tmav\u00e9m a such\u00e9m m\u00edst\u011b. D\u00e1le plat\u00ed obvykl\u00e1 laboratorn\u00ed opat\u0159en\u00ed: pracujte v \u010dist\u00e9m a such\u00e9m prost\u0159ed\u00ed, p\u0159ed otev\u0159en\u00edm nechte lahvi\u010dku dos\u00e1hnout pokojov\u00e9 teploty, aby se zabr\u00e1nilo kondenzaci, a do laboratorn\u00edho protokolu zaznamenejte \u010d\u00edslo \u0161ar\u017ee a datum otev\u0159en\u00ed ka\u017ed\u00e9 lahvi\u010dky, aby byla n\u00e1sledn\u011b sledovateln\u00e1 s\u00e9rie m\u011b\u0159en\u00ed.<\/p>\n<h2>Analytick\u00e1 kvalita<\/h2>\n<p>U tripeptidu je mal\u00fd prostor pro nejistotu ohledn\u011b v\u00fdchoz\u00ed l\u00e1tky: vedlej\u0161\u00ed produkty synt\u00e9zy a voln\u00e9 aminokyseliny se molekulovou hmotnost\u00ed bl\u00ed\u017e\u00ed hlavn\u00ed slo\u017ece a mohou se shodovat s hlavn\u00edm p\u00edkem v chromatogramu. N\u00e1\u0161 KPV se dod\u00e1v\u00e1 s \u010distotou \u2265 99 %, stanovenou HPLC anal\u00fdzou v souladu s odpov\u00eddaj\u00edc\u00edm analytick\u00fdm certifik\u00e1tem. Dostupn\u00e9 analytick\u00e9 certifik\u00e1ty si m\u016f\u017eete prohl\u00e9dnout na na\u0161ich webov\u00fdch str\u00e1nk\u00e1ch. <a href=\"https:\/\/lumopeptides.com\/labresultaten\/\" target=\"_blank\" rel=\"noopener\">str\u00e1nka s v\u00fdsledky laboratorn\u00edch test\u016f<\/a>.<\/p>\n<h2>D\u016fle\u017eit\u00e9 upozorn\u011bn\u00ed<\/h2>\n<p>Tento produkt dod\u00e1v\u00e1me v\u00fdhradn\u011b pro laboratorn\u00ed v\u00fdzkum a analytick\u00e9 pr\u00e1ce. Nen\u00ed ur\u010den pro diagnostick\u00e9 ani terapeutick\u00e9 aplikace a v\u00fdslovn\u011b nen\u00ed ur\u010den k pou\u017eit\u00ed u lid\u00ed nebo zv\u00ed\u0159at. Tento produkt nen\u00ed potravinou, dopl\u0148kem stravy, kosmetick\u00fdm p\u0159\u00edpravkem ani registrovan\u00fdm l\u00e9\u010div\u00fdm p\u0159\u00edpravkem. Zakoupen\u00edm tohoto produktu kupuj\u00edc\u00ed potvrzuje, \u017ee je star\u0161\u00ed 18 let a bude jej pou\u017e\u00edvat v\u00fdhradn\u011b v kontrolovan\u00e9m v\u00fdzkumn\u00e9m nebo laboratorn\u00edm prost\u0159ed\u00ed.<\/p>","protected":false},"excerpt":{"rendered":"<p>KPV je syntetick\u00fd tripeptid se sekvenc\u00ed Lys-Pro-Val (C<sub>16<\/sub>H<sub>30<\/sub>N<sub>4<\/sub>O<sub>4<\/sub>, p\u0159ibli\u017en\u011b 342,44 Da), ve kter\u00e9m centr\u00e1ln\u00ed prolin omezuje rota\u010dn\u00ed volnost kolem sousedn\u00ed peptidov\u00e9 vazby. V r\u00e1mci v\u011bdeck\u00e9ho v\u00fdzkumu je studov\u00e1n jako modelov\u00e1 slou\u010denina v peptidov\u00e9 chemii a analytick\u00fdch prac\u00edch. Ur\u010deno v\u00fdhradn\u011b pro laboratorn\u00ed a v\u00fdzkumn\u00e9 \u00fa\u010dely; nikoli pro pou\u017eit\u00ed u lid\u00ed nebo zv\u00ed\u0159at. Nen\u00ed to l\u00e9\u010div\u00fd p\u0159\u00edpravek.<\/p>","protected":false},"featured_media":3228,"comment_status":"open","ping_status":"closed","template":"","meta":[],"product_brand":[],"product_cat":[125,121],"product_tag":[],"class_list":["post-2905","product","type-product","status-publish","has-post-thumbnail","product_cat-herstel-reparatie","product_cat-peptides","first","instock","shipping-taxable","purchasable","product-type-variable"],"_links":{"self":[{"href":"https:\/\/pevropeptides.com\/cs\/wp-json\/wp\/v2\/product\/2905","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pevropeptides.com\/cs\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/pevropeptides.com\/cs\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/pevropeptides.com\/cs\/wp-json\/wp\/v2\/comments?post=2905"}],"version-history":[{"count":1,"href":"https:\/\/pevropeptides.com\/cs\/wp-json\/wp\/v2\/product\/2905\/revisions"}],"predecessor-version":[{"id":2985,"href":"https:\/\/pevropeptides.com\/cs\/wp-json\/wp\/v2\/product\/2905\/revisions\/2985"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/pevropeptides.com\/cs\/wp-json\/wp\/v2\/media\/3228"}],"wp:attachment":[{"href":"https:\/\/pevropeptides.com\/cs\/wp-json\/wp\/v2\/media?parent=2905"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/pevropeptides.com\/cs\/wp-json\/wp\/v2\/product_brand?post=2905"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/pevropeptides.com\/cs\/wp-json\/wp\/v2\/product_cat?post=2905"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/pevropeptides.com\/cs\/wp-json\/wp\/v2\/product_tag?post=2905"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}